9AU4

Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain

これはPDB形式変換不可エントリーです。

9AU4 の概要

• エントリーDOI: 10.2210/pdb9au4/pdb
• EMDBエントリー: 43843
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII, ... (27 entities in total)
• 機能のキーワード: cyanobacteria, photosystem i, menb, phylloquinone, plastoquinone, benzoquinone, naphthoquinone, redox potential, photosynthesis
• 由来する生物種: Synechocystis sp. PCC 6803; 詳細
• タンパク質・核酸の鎖数: 33
• 化学式量合計: 1077744.42

構造登録者

Gisriel, C.J.,Vasily, K.,Iwig, D.F.,Russell, B.P.,Vinyard, D.J.,Golbeck, J.H.,Brudvig, G.W.,Lakshmi, K.V. (登録日: 2024-02-28, 公開日: 2024-12-25)

主引用文献

Gisriel, C.J.,Kurashov, V.,Iwig, D.F.,Russell, B.P.,Vinyard, D.J.,Brudvig, G.W.,Golbeck, J.H.,Lakshmi, K.V.
Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain.
Sci Adv, 10:eadp4937-eadp4937, 2024

PubMed Abstract: Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB deletion (Δ) variant in the cyanobacterium sp. PCC 6803, in which phylloquinone is replaced by plastoquinone-9 with a low binding affinity. This permits its exchange with exogenous quinones covalently coupled to dihydrogen catalysts that bind with high affinity, thereby converting PS I into a stable solar fuel catalyst. Here, we reveal the 2.03-Å-resolution cryo-EM structure of a recent MenB variant of PS I. The quinones and their binding environment are analyzed in the context of previous biophysical data, thereby enabling a protocol to solve future PS I hybrids and constructs from this genetically tractable cyanobacterium.

PubMed: 39612342
DOI: 10.1126/sciadv.adp4937

実験手法

ELECTRON MICROSCOPY (2.03 Å)