9ATW

Structure of biofilm-forming functional amyloid PSMa1 from Staphylococcus aureus

これはPDB形式変換不可エントリーです。

9ATW の概要

• エントリーDOI: 10.2210/pdb9atw/pdb
• EMDBエントリー: 43835
• 分子名称: Phenol-soluble modulin alpha 1 peptide (1 entity in total)
• 機能のキーワード: functional amyloid fibril, biofilm, bacterial biofilm, phenol soluble modulin alpha1, psma1, structural protein
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 64
• 化学式量合計: 144820.29

構造登録者

Hansen, K.H.,Byeon, C.H.,Liu, Q.,Drace, T.,Boesen, T.,Conway, J.F.,Andreasen, M.,Akbey, U. (登録日: 2024-02-27, 公開日: 2024-08-07, 最終更新日: 2024-08-21)

主引用文献

Hansen, K.H.,Byeon, C.H.,Liu, Q.,Drace, T.,Boesen, T.,Conway, J.F.,Andreasen, M.,Akbey, U.
Structure of biofilm-forming functional amyloid PSM alpha 1 from Staphylococcus aureus.
Proc.Natl.Acad.Sci.USA, 121:e2406775121-e2406775121, 2024

PubMed Abstract: Biofilm-protected pathogenic causes chronic infections that are difficult to treat. An essential building block of these biofilms are functional amyloid fibrils that assemble from phenol-soluble modulins (PSMs). PSMα1 cross-seeds other PSMs into cross-β amyloid folds and is therefore a key element in initiating biofilm formation. However, the paucity of high-resolution structures hinders efforts to prevent amyloid assembly and biofilm formation. Here, we present a 3.5 Å resolution density map of the major PSMα1 fibril form revealing a left-handed cross-β fibril composed of two C-symmetric U-shaped protofilaments whose subunits are unusually tilted out-of-plane. Monomeric α-helical PSMα1 is extremely cytotoxic to cells, despite the moderate toxicity of the cross-β fibril. We suggest mechanistic insights into the PSM functional amyloid formation and conformation transformation on the path from monomer-to-fibril formation. Details of PSMα1 assembly and fibril polymorphism suggest how utilizes functional amyloids to form biofilms and establish a framework for developing therapeutics against infection and antimicrobial resistance.

PubMed: 39116134
DOI: 10.1073/pnas.2406775121

実験手法

ELECTRON MICROSCOPY (3.5 Å)