8ZTP

Crystal structure of cysteine desulfurase Sufs from Mycoplasma Pneumonia

8ZTP の概要

• エントリーDOI: 10.2210/pdb8ztp/pdb
• 分子名称: cysteine desulfurase, PYRIDOXAL-5'-PHOSPHATE (2 entities in total)
• 機能のキーワード: sufs, mycoplasma pneumonia, plp-binding, l-cys, biosynthetic protein
• 由来する生物種: Mycoplasmoides pneumoniae 19294
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95576.71

構造登録者

Wang, W.M.,Ma, D.Y.,Gong, W.J.,Yao, H.,Liu, Y.H.,Wang, H.F. (登録日: 2024-06-07, 公開日: 2024-12-11, 最終更新日: 2024-12-18)

主引用文献

Ma, D.,Yao, H.,Liu, Y.,Gong, W.,Zhao, Y.,Wang, R.,Wu, C.,Wang, W.,Wang, H.
The reduced interaction between SufS and SufU in Mycoplasma penetrans results in diminished sulfotransferase activity.
Int.J.Biol.Macromol., 284:138181-138181, 2024

PubMed Abstract: Mycoplasma Penetrans (Mpe) is an AIDS-related mycoplasma that is also closely related to respiratory diseases. Proteins involved in the first phase of Fe-S cluster biosynthesis in the SUF-like pathway are essential in Gram-positive bacteria because there is no redundant Fe-S cluster biosynthetic pathway in these proteins. In this study, we characterized two essential proteins: cysteine desulphurase (MpeSufS) and sulfurtransferase (MpeSufU) in Mpe, and resolved their crystal structures. Our results reveal that MpeSufS belongs to type II cysteine desulfurase, and MpeSufU is a Zn-containing sulfurtransferase. Residue Q342 in MpeSufS and the zinc atom in MpeSufU mediate sulfur transfer from MpeSufS to MpeSufU. Mutation of Q342 significantly impacts the cysteine desulfurase activity. This study provides new insights into the regulation of the activity of the SufS-SufU complex, which will help guide the design of drugs for the treatment of mycoplasma infections.

PubMed: 39615726
DOI: 10.1016/j.ijbiomac.2024.138181

実験手法

X-RAY DIFFRACTION (3.2 Å)