8ZTL
AtALMT9 with LMNG (intermediate class)
Summary for 8ZTL
| Entry DOI | 10.2210/pdb8ztl/pdb |
| EMDB information | 60466 |
| Descriptor | Aluminum-activated malate transporter 9, PALMITIC ACID, 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE, ... (6 entities in total) |
| Functional Keywords | plant, stomata, vacuole, almt, ion channel, membrane protein |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 2 |
| Total formula weight | 135680.12 |
| Authors | |
| Primary citation | Lee, Y.,Demes-Causse, E.,Yoo, J.,Jang, S.Y.,Jung, S.,Jaslan, J.,Hwang, G.S.,Yoo, J.,De Angeli, A.,Lee, S. Structural basis for malate-driven, pore lipid-regulated activation of the Arabidopsis vacuolar anion channel ALMT9. Nat Commun, 16:1817-1817, 2025 Cited by PubMed Abstract: In plant cells, ALMTs are key plasma and vacuolar membrane-localized anion channels regulating plant responses to the environment. Vacuolar ALMTs control anion accumulation in plant cells and, in guard cells, they regulate stomata aperture. The activation of vacuolar ALMTs depends on voltage and cytosolic malate, but the underlying molecular mechanisms remain elusive. Here we report the cryo-EM structures of ALMT9 from Arabidopsis thaliana (AtALMT9), a malate-activated vacuolar anion channel, in plugged and unplugged lipid-bound states. In all these states, membrane lipids interact with the ion conduction pathway of AtALMT9. We identify two unplugged states presenting two distinct pore width profiles. Combining structural and functional analysis we identified conserved residues involved in ion conduction and in the pore lipid interaction. Molecular dynamics simulations revealed a peculiar anion conduction mechanism in AtALMT9. We propose a voltage-dependent activation mechanism based on the competition between pore lipids and malate at the cytosolic entrance of the channel. PubMed: 39979303DOI: 10.1038/s41467-025-56940-5 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.75 Å) |
Structure validation
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