8ZT4

Complex structure of N-acetyltransferase SbzI and carrier protein SbzG in the biosynthesis of altemicidin

8ZT4 の概要

• エントリーDOI: 10.2210/pdb8zt4/pdb
• 分子名称: Carrier protein,GNAT family transferase, THIOCYANATE ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: a general control non-repressible 5 (gcn5)-related n-acetyltransferase, altemicidin, biosynthesis, transferase
• 由来する生物種: Streptomyces sp.; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64172.30

構造登録者

Zhu, Y.,Mori, T.,Abe, I. (登録日: 2024-06-06, 公開日: 2025-02-05)

主引用文献

Zhu, Y.,Mori, T.,Karasawa, M.,Shirai, K.,Cheng, W.,Terada, T.,Awakawa, T.,Abe, I.
Structure-function analysis of carrier protein-dependent 2-sulfamoylacetyl transferase in the biosynthesis of altemicidin.
Nat Commun, 15:10896-10896, 2024

PubMed Abstract: The general control non-repressible 5 (GCN5)-related N-acetyltransferase (GNAT) SbzI, in the biosynthesis of the sulfonamide antibiotic altemicidin, catalyzes the transfer of the 2-sulfamoylacetyl (2-SA) moiety onto 6-azatetrahydroindane dinucleotide. While most GNAT superfamily utilize acyl-coenzyme A (acyl-CoA) as substrates, SbzI recognizes a carrier-protein (CP)-tethered 2-SA substrate. Moreover, SbzI is the only naturally occurring enzyme that catalyzes the direct incorporation of sulfonamide, a valuable pharmacophore in medicinal chemistry. Here, we present the structure-function analysis and structure-based engineering of SbzI. The crystal structure of SbzI in complex with the CP SbzG, along with cross-linking and isothermal titration calorimetry analyses of their variants, revealed the structural basis for CP recognition by the GNAT SbzI. Furthermore, docking simulation, molecular dynamics simulation, and mutagenesis studies indicated the intimate structural details of the unique reaction mechanism of SbzI, which does not utilize a general base residue in contrast to other typical GNATs. These findings facilitated rational engineering of the enzyme to expand the substrate range and to generate azaindane dinucleotide derivatives.

PubMed: 39738057
DOI: 10.1038/s41467-024-55265-z

実験手法

X-RAY DIFFRACTION (2.6 Å)