8ZSU

Lysozyme Crystallized with Bioassembler in Space Microgravity

Replaces:  8RT3

Summary for 8ZSU

• Entry DOI: 10.2210/pdb8zsu/pdb
• Descriptor: Lysozyme C, GADOLINIUM ATOM, 10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE 1,4,7-TRIACETIC ACID, ... (7 entities in total)
• Functional Keywords: lysozyme, gadolinium complex, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 15752.29

Authors

MacCarthy, C.,Koudan, E.V.,Petrov, S.V.,Levin, A.A.,Khesuani, Y.D.,Borshchevskiy, V. (deposition date: 2024-06-05, release date: 2025-05-28, Last modification date: 2025-06-25)

Primary citation

MacCarthy, C.,Koudan, E.,Shevtsov, M.,Parfenov, V.,Petrov, S.,Levin, A.,Senatov, F.,Sykilinda, N.,Ostrovskiy, S.,Pekov, S.,Gushchin, I.,Popov, I.,Zinovev, E.,Bogorodskiy, A.,Mishin, A.,Ivanovich, V.,Rogachev, A.,Khesuani, Y.,Borshchevskiy, V.
Exploring the potential of a bioassembler for protein crystallization in space.
Npj Microgravity, 11:25-25, 2025

PubMed Abstract: Protein crystallization holds paramount significance in structural biology, serving as a pivotal technique for unveiling the three-dimensional (3D) architecture of proteins. While microgravity conditions in space offer distinct advantages for high-quality protein crystal growth by mitigating the influences of gravity and convection, the development of reliable techniques for protein crystallization in space with precise control over the crystallization process and its meticulous inspections remains a challenge. In this study, we present an innovative bioassembler-specifically, the 'Organ.Aut'-which we successfully employed to crystallize protein in space. The bioassembler 'Organ.Aut' produced highly ordered crystals diffracted to a true-atomic resolution of ∼1 Å. These data allowed for a detailed examination of atomic structures, enabling thorough structural comparisons with crystals grown on Earth. Our finding suggests that the bioassembler 'Organ.Aut' stands as a promising and viable option for advancing protein crystallization in space.

PubMed: 40517138
DOI: 10.1038/s41526-025-00477-w

Experimental method

X-RAY DIFFRACTION (1.09 Å)