8ZSU

Lysozyme Crystallized with Bioassembler in Space Microgravity

「8RT3」から置き換えられました

8ZSU の概要

• エントリーDOI: 10.2210/pdb8zsu/pdb
• 分子名称: Lysozyme C, GADOLINIUM ATOM, 10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE 1,4,7-TRIACETIC ACID, ... (7 entities in total)
• 機能のキーワード: lysozyme, gadolinium complex, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15752.29

構造登録者

MacCarthy, C.,Koudan, E.V.,Petrov, S.V.,Levin, A.A.,Khesuani, Y.D.,Borshchevskiy, V. (登録日: 2024-06-05, 公開日: 2025-05-28, 最終更新日: 2025-06-25)

主引用文献

MacCarthy, C.,Koudan, E.,Shevtsov, M.,Parfenov, V.,Petrov, S.,Levin, A.,Senatov, F.,Sykilinda, N.,Ostrovskiy, S.,Pekov, S.,Gushchin, I.,Popov, I.,Zinovev, E.,Bogorodskiy, A.,Mishin, A.,Ivanovich, V.,Rogachev, A.,Khesuani, Y.,Borshchevskiy, V.
Exploring the potential of a bioassembler for protein crystallization in space.
Npj Microgravity, 11:25-25, 2025

PubMed Abstract: Protein crystallization holds paramount significance in structural biology, serving as a pivotal technique for unveiling the three-dimensional (3D) architecture of proteins. While microgravity conditions in space offer distinct advantages for high-quality protein crystal growth by mitigating the influences of gravity and convection, the development of reliable techniques for protein crystallization in space with precise control over the crystallization process and its meticulous inspections remains a challenge. In this study, we present an innovative bioassembler-specifically, the 'Organ.Aut'-which we successfully employed to crystallize protein in space. The bioassembler 'Organ.Aut' produced highly ordered crystals diffracted to a true-atomic resolution of ∼1 Å. These data allowed for a detailed examination of atomic structures, enabling thorough structural comparisons with crystals grown on Earth. Our finding suggests that the bioassembler 'Organ.Aut' stands as a promising and viable option for advancing protein crystallization in space.

PubMed: 40517138
DOI: 10.1038/s41526-025-00477-w

実験手法

X-RAY DIFFRACTION (1.09 Å)