8ZNJ

Cryo-EM structure of a short prokaryotic Argonaute system from archaeon Suldolobus islandicus

8ZNJ の概要

• エントリーDOI: 10.2210/pdb8znj/pdb
• EMDBエントリー: 60273
• 分子名称: Piwi domain-containing protein, SiAgo-associated protein1, SiAga1, RNA (5'-R(P*UP*CP*AP*AP*AP*GP*CP*UP*UP*AP*GP*AP*UP*AP*CP*CP*CP*UP*GP*GP*A)-3'), ... (5 entities in total)
• 機能のキーワード: argonaute, rna binding protein/rna/dna, rna binding protein-rna-dna complex
• 由来する生物種: Saccharolobus islandicus M.16.4; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95941.13

構造登録者

Dai, Z.K.,Guan, Z.Y.,Han, W.Y.,Zou, T.T. (登録日: 2024-05-27, 公開日: 2025-02-19, 最終更新日: 2025-07-23)

主引用文献

Dai, Z.,Chen, Y.,Guan, Z.,Chen, X.,Tan, K.,Yang, K.,Yan, X.,Liu, Y.,Gong, Z.,Han, W.,Zou, T.
Structural and mechanistic insights into the activation of a short prokaryotic argonaute system from archaeon Sulfolobus islandicus.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Prokaryotic Argonaute proteins (pAgos) defend the host against invading nucleic acids, including plasmids and viruses. Short pAgo systems confer immunity by inducing cell death upon detecting invading nucleic acids. However, the activation mechanism of the SiAgo system, comprising a short pAgo from the archaeon Sulfolobus islandicus and its associated proteins SiAga1 and SiAga2, remains largely unknown. Here, we determined the cryo-electron microscopy structures of the SiAgo-Aga1 apo complex and the RNA-DNA-bound SiAgo-Aga1 complex at resolutions of 2.7 and 3.0 Å, respectively. Our results revealed that a positively charged pocket is generated from the interaction between SiAgo and SiAga1, exhibiting an architecture similar to APAZ-pAgo of short pAgo systems and accommodating the nucleic acids. Further investigation elucidated the conserved mechanism of nucleic acid recognition by SiAgo-Aga1. Both the SiAgo-Aga1 interaction and nucleic acid recognition by the complex are essential for antiviral defense. Biochemical and structural analyses demonstrated that SiAgo-Aga1 undergoes extensive conformational changes upon binding to the RNA-DNA duplex, thereby licensing its interaction with the effector SiAga2 to trigger the immune response. Overall, our findings highlight the evolutionary conservation of Agos across phylogenetic clades and provide structural insights into the activation mechanism of the SiAgo system.

PubMed: 39898546
DOI: 10.1093/nar/gkaf059

実験手法

ELECTRON MICROSCOPY (3 Å)