8ZJW

Cryo-EM structure of photosynthetic LH1' complex of Roseospirillum parvum

8ZJW の概要

• エントリーDOI: 10.2210/pdb8zjw/pdb
• EMDBエントリー: 60158
• 分子名称: Beta subunit of light-harvesting 1 complex, Alpha subunit of light-harvesting 1 complex, BACTERIOCHLOROPHYLL A, ... (4 entities in total)
• 機能のキーワード: lh1 prime complex, roseospirillum parvum 930i, photosynthesis
• 由来する生物種: Roseospirillum parvum; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 251991.29

構造登録者

Wang, G.-L.,Wang, X.-P.,Yu, L.-J. (登録日: 2024-05-15, 公開日: 2025-01-22)

主引用文献

Wang, X.P.,Wang, G.L.,Fu, Y.,Minamino, A.,Zou, M.J.,Ma, F.,Xu, B.,Wang-Otomo, Z.Y.,Kimura, Y.,Madigan, M.T.,Overmann, J.,Yu, L.J.
Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum.
Commun Biol, 7:1658-1658, 2024

PubMed Abstract: Purple phototrophic bacteria produce two kinds of light-harvesting complexes that function to capture and transmit solar energy: the core antenna (LH1) and the peripheral antenna (LH2). The apoproteins of these antennas, encoded respectively by the genes pufBA and pucBA within and outside the photosynthetic gene cluster, respectively, exhibit conserved amino acid sequences and structural topologies suggesting they were derived from a shared ancestor. Here we present the structures of two photosynthetic complexes from Roseospirillum (Rss.) parvum 930I: an LH1-RC complex and a variant of the LH1 complex also encoded by pufBA that we designate as LH1'. The LH1-RC complex forms a closed elliptical structure consisting of 16 pairs of αβ-polypeptides that surrounds the RC. By contrast, the LH1' complex is a closed ring structure composed of 14 pairs of αβ-polypeptides, and it shows significant similarities to LH2 complexes both spectrally and structurally. Although LH2-like, the LH1' complex is larger than any known LH2 complexes, and genomic analyses of Rss. parvum revealed the absence of pucBA, genes that encode classical LH2 complexes. Characterization of the unique Rss. parvum photocomplexes not only underscores the diversity of such structures but also sheds new light on the evolution of light-harvesting complexes from phototrophic bacteria.

PubMed: 39702771
DOI: 10.1038/s42003-024-07354-4

実験手法

ELECTRON MICROSCOPY (2.35 Å)