8ZF1

Crystal structure of a Chemo Triplet Photoenzyme (CTPe)

8ZF1 の概要

• エントリーDOI: 10.2210/pdb8zf1/pdb
• 分子名称: Transcriptional regulator, PadR-like family, N-(9-oxidanylidenethioxanthen-2-yl)ethanamide (2 entities in total)
• 機能のキーワード: aritificial enzyme, protein modification, biocatalysis, energy transfer, photosynthesis
• 由来する生物種: Lactococcus lactis subsp. cremoris (strain MG1363)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15280.23

構造登録者

Chen, X.,Qian, J.Y.,Guo, J.,Wu, Y.Z.,Zhong, F.R. (登録日: 2024-05-07, 公開日: 2024-07-17, 最終更新日: 2024-11-13)

主引用文献

Guo, J.,Qian, J.,Cai, D.,Huang, J.,Yang, X.,Sun, N.,Zhang, J.,Pang, T.,Zhao, W.,Wu, G.,Chen, X.,Zhong, F.,Wu, Y.
Chemogenetic Evolution of Diversified Photoenzymes for Enantioselective [2 + 2] Cycloadditions in Whole Cells.
J.Am.Chem.Soc., 146:19030-19041, 2024

PubMed Abstract: Artificial photoenzymes with novel catalytic modes not found in nature are in high demand; yet, they also present significant challenges in the field of biocatalysis. In this study, a chemogenetic modification strategy is developed to facilitate the rapid diversification of photoenzymes. This strategy integrates site-specific chemical conjugation of various artificial photosensitizers into natural protein cavities and the iterative mutagenesis in cell lysates. Through rounds of directed evolution, prominent visible-light-activatable photoenzyme variants were developed, featuring a thioxanthone chromophore. They successfully enabled the enantioselective [2 + 2] photocycloaddition of 2-carboxamide indoles, a class of UV-sensitive substrates that are traditionally challenging for known photoenzymes. Furthermore, the versatility of this photoenzyme is demonstrated in enantioselective whole-cell photobiocatalysis, enabling the efficient synthesis of enantioenriched cyclobutane-fused indoline tetracycles. These findings significantly expand the photophysical properties of artificial photoenzymes, a critical factor in enhancing their potential for harnessing excited-state reactivity in stereoselective transformations.

PubMed: 38976645
DOI: 10.1021/jacs.4c03087

実験手法

X-RAY DIFFRACTION (2.6 Å)