8ZEK

Cryo-EM structure of the E. coli BrxX methyltransferase complexed with Ocr

8ZEK の概要

• エントリーDOI: 10.2210/pdb8zek/pdb
• EMDBエントリー: 60037
• 分子名称: site-specific DNA-methyltransferase (adenine-specific), Protein Ocr (2 entities in total)
• 機能のキーワード: methyltransferase, complex, transferase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 165681.25

構造登録者

Zhu, L.,Xu, T.H.,Sun, L.T. (登録日: 2024-05-06, 公開日: 2024-12-11, 最終更新日: 2025-07-16)

主引用文献

Li, S.,Xu, T.,Meng, X.,Yan, Y.,Zhou, Y.,Duan, L.,Tang, Y.,Zhu, L.,Sun, L.
Ocr-mediated suppression of BrxX unveils a phage counter-defense mechanism.
Nucleic Acids Res., 52:8580-8594, 2024

PubMed Abstract: The burgeoning crisis of antibiotic resistance has directed attention to bacteriophages as natural antibacterial agents capable of circumventing bacterial defenses. Central to this are the bacterial defense mechanisms, such as the BREX system, which utilizes the methyltransferase BrxX to protect against phage infection. This study presents the first in vitro characterization of BrxX from Escherichia coli, revealing its substrate-specific recognition and catalytic activity. We demonstrate that BrxX exhibits nonspecific DNA binding but selectively methylates adenine within specific motifs. Kinetic analysis indicates a potential regulation of BrxX by the concentration of its co-substrate, S-adenosylmethionine, and suggests a role for other BREX components in modulating BrxX activity. Furthermore, we elucidate the molecular mechanism by which the T7 phage protein Ocr (Overcoming classical restriction) inhibits BrxX. Despite low sequence homology between BrxX from different bacterial species, Ocr effectively suppresses BrxX's enzymatic activity through high-affinity binding. Cryo-electron microscopy and biophysical analyses reveal that Ocr, a DNA mimic, forms a stable complex with BrxX, highlighting a conserved interaction interface across diverse BrxX variants. Our findings provide insights into the strategic counteraction by phages against bacterial defense systems and offer a foundational understanding of the complex interplay between phages and their bacterial hosts, with implications for the development of phage therapy to combat antibiotic resistance.

PubMed: 38989624
DOI: 10.1093/nar/gkae608

実験手法

ELECTRON MICROSCOPY (3.15 Å)