8ZDL

Cryo-EM structure of Mycobacteriophage Douge genome-free connector (gp5, gp9, gp10, gp12 and gp13)

8ZDL の概要

• エントリーDOI: 10.2210/pdb8zdl/pdb
• EMDBエントリー: 39989 39991 39992 39994 60715
• 分子名称: Protal Protein (gp5), Stopper Protein (gp10), Tail Tube Protein (gp13), ... (5 entities in total)
• 機能のキーワード: mycobacteriophage, phage structure, icosahedral, cryo-em, protein assembly, virus, viral protein
• 由来する生物種: Mycolicibacterium smegmatis MC2 155; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 1313272.38

構造登録者

Maharana, J.,Wang, C.H.,Tsai, L.A.,Lowary, T.L.,Ho, M.C. (登録日: 2024-05-02, 公開日: 2025-06-25, 最終更新日: 2025-09-03)

主引用文献

Maharana, J.,Wang, C.H.,Tsai, L.A.,Liao, Y.T.,Yang, C.H.,Shen, M.C.,Macale, L.S.,Tran, T.N.,Narsico, J.,Perez, R.J.,Tewary, S.K.,Wu, J.L.,Lin, H.Y.,Chang, S.W.,Franklin, A.,Moynihan, P.J.,Jacobs-Sera, D.,Freeman, K.G.,Hatfull, G.F.,Lowary, T.L.,Ho, M.C.
Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge.
Cell Rep, 44:116057-116057, 2025

PubMed Abstract: Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering to increase efficacy and broaden host range, but such information is currently unavailable. We describe the cryoelectron microscopy (cryo-EM) structure of mycobacteriophage Douge, which contains 1,105 protein subunits assembled into a complete siphophage and is coated with glycan-binding domains for mycobacterial cell surface interactions. When filled with viral genome, the channel spanning the connector, tail, and baseplate is sealed by tape measure proteins, providing a genome gating system and requiring limited structural changes for genome ejection upon phage-host contact. Nanometer-resolution cryoelectron tomography (cryo-ET) snapshots of phage-host interactions show that the baseplate remains attached to the mycobacterial outer membrane during viral genome ejection. This study reveals high-resolution structural details of this mycobacteriophage and its interaction with host glycans.

PubMed: 40711877
DOI: 10.1016/j.celrep.2025.116057

実験手法

ELECTRON MICROSCOPY (3.78 Å)