8ZAJ
Cryo-EM structure of a 55 kDa nucleoplasmin domain of AtFKBP53
Summary for 8ZAJ
| Entry DOI | 10.2210/pdb8zaj/pdb |
| EMDB information | 39878 |
| Descriptor | Peptidyl-prolyl cis-trans isomerase FKBP53 (1 entity in total) |
| Functional Keywords | nucleoplasmin domain, chaperon, chaperone |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 5 |
| Total formula weight | 60558.04 |
| Authors | Bharambe, N.,Saharan, K.,Vasudevan, D.,Basak, S. (deposition date: 2024-04-25, release date: 2025-05-14) |
| Primary citation | Bharambe, N.,Saharan, K.,Vasudevan, D.,Basak, S. 2.0 angstrom cryo-EM structure of the 55 kDa nucleoplasmin domain of AtFKBP53. J.Struct.Biol., 217:108203-108203, 2025 Cited by PubMed Abstract: The knowledge of three-dimensional structures of biological macromolecules is crucial for understanding the molecular mechanisms underlying disease pathology and for devising drugs targeting specific molecules. Single particle cryo-electron microscopy (Cryo-EM) has become indispensable for this purpose, particularly for large macromolecules and their complexes. However, its effectiveness has been limited in achieving near-atomic resolution for smaller macromolecules. This study presents the Cryo-EM structure of a 55 kDa pentameric AtFKBP53 nucleoplasmin domain at 2.0 Å nominal resolution. Our approach involves selecting the optimal grid for data collection and precise alignment of small particles to enhance the resolution of the final 3D reconstructed map. In this study, we systematically processed cryo-EM dataset of a small molecule to improve alignment, and this data processing strategy can be used as a guidance to process the cryo-EM data of other small molecules. PubMed: 40262726DOI: 10.1016/j.jsb.2025.108203 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2 Å) |
Structure validation
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