8ZAG

Crystal structure of SkABA3 from Shimazuella kribbensis in complex with PPi

8ZAG の概要

• エントリーDOI: 10.2210/pdb8zag/pdb
• 分子名称: Sesquiterpene synthases, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: skaba3, sesquiterpene synthases, ppi, lyase
• 由来する生物種: Shimazuella kribbensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47794.62

構造登録者

Li, S.Y.,Li, H.,Yang, Y.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T. (登録日: 2024-04-25, 公開日: 2024-12-11, 最終更新日: 2025-01-22)

主引用文献

Li, S.,Huang, J.W.,Min, J.,Li, H.,Ning, M.,Zhou, S.,Yang, Y.,Chen, C.C.,Guo, R.T.
Molecular insights into a distinct class of terpenoid cyclases.
Nat Commun, 16:207-207, 2025

PubMed Abstract: Terpenoid cyclases (TCs) account for the synthesis of the most widespread and diverse natural compounds. A sesquiterpene cyclase termed BcABA3 from an abscisic acid-producing fungus Botrytis cinerea that yields (2Z,4E)-α-ionylideneethane but lacks signature feature of canonical TCs represents a distinct type of TCs. Here, we report the crystal structures of BcABA3, a closely related RuABA3 from Rutstroemia sp. and a bacterial SkABA3 from Shimazuella kribbensis. These ABA3 proteins adopt an all-α-helix fold and bind pyrophosphate moiety of farnesyl pyrophosphate by Glu-chelated Mg ion cluster. We conduct mutagenesis experiments to validate the role of the substrate-binding residues. SkABA3 appears to yield compounds that are distinct from (2Z,4E)-α-ionylideneethane. These results not only provide the molecular insight into ABA3 proteins that serve as an important basis to the future investigation of this class of TCs, but also reveal the existence of more uncharacterized terpenoids synthesized via dedicated machineries.

PubMed: 39747870
DOI: 10.1038/s41467-024-55717-6

実験手法

X-RAY DIFFRACTION (1.6 Å)