8Z77

The structure of thiocyanate dehydrogenase from Pelomicrobium methylotrophicum (pmTcDH), activated by crystals soaking with 1 mM CuCl2 and Na ascorbate during 12 hours

Summary for 8Z77

• Entry DOI: 10.2210/pdb8z77/pdb
• Related: 8Z75
• Descriptor: Twin-arginine translocation signal domain-containing protein, COPPER (II) ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: thiocyanate dehydrogenase, copper incorporation in the active site, copper enzyme, trinuclear copper center, activation by crystals soaking, pmtcdh, pelomicrobium methylotrophicum, conformational changes in a crystal, oxidoreductase
• Biological source: Pelomicrobium methylotrophicum
• Total number of polymer chains: 3
• Total formula weight: 162975.95

Authors

Varfolomeeva, L.A.,Solovieva, A.Y.,Shipkov, N.S.,Dergousova, N.I.,Minyaev, M.E.,Boyko, K.M.,Tikhonova, T.V.,Popov, V.O. (deposition date: 2024-04-19, release date: 2024-05-08, Last modification date: 2025-05-21)

• Primary citation: Varfolomeeva, L.A.,Solovieva, A.Y.,Shipkov, N.S.,Dergousova, N.I.,Minyaev, M.E.,Boyko, K.M.,Tikhonova, T.V.,Popov, V.O.; Effect of Copper Ions on the Crystal Packing and Conformation of Thiocyanate Dehydrogenase in the Crystal Structure; Crystallography Reports, 70:8-15, 2025; DOI: 10.1134/S1063774524602478

Experimental method

X-RAY DIFFRACTION (2 Å)