8Z4E

Structure of human 18_14D scFv and 1G01 scFv in complex with influenza virus neuraminidase from A/Yunan/DQ001/2016 (H5N6)

8Z4E の概要

• エントリーDOI: 10.2210/pdb8z4e/pdb
• EMDBエントリー: 39762
• 分子名称: Neuraminidase, Light chain of 18_14D scFv, Heavy chain of 18_14D scFv, ... (7 entities in total)
• 機能のキーワード: influenza virus, neuraminidase, human monoclonal antibody, immunogen, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 139108.46

構造登録者

Wang, M.,Peng, Q.,Gao, Y.,Shi, Y. (登録日: 2024-04-17, 公開日: 2025-01-22)

主引用文献

Wang, M.,Gao, Y.,Shen, C.,Yang, W.,Peng, Q.,Cheng, J.,Shen, H.M.,Yang, Y.,Gao, G.F.,Shi, Y.
A human monoclonal antibody targeting the monomeric N6 neuraminidase confers protection against avian H5N6 influenza virus infection.
Nat Commun, 15:8871-8871, 2024

PubMed Abstract: The influenza neuraminidase (NA) is a potential target for the development of a next-generation influenza vaccine, but its antigenicity is not well understood. Here, we isolate an anti-N6 human monoclonal antibody, named 18_14D, from an H5N6 avian influenza virus (AIV) infected patient. The antibody weakly inhibits enzymatic activity but confers protection in female mice, mainly via ADCC function. The cryo-EM structure shows that 18_14D binds to a unique epitope on the lateral surface of the N6 tetramer, preventing the formation of tightly closed NA tetramers. These findings contribute to the molecular understanding of protective immune responses to NA of AIVs in humans and open an avenue for the rational design of NA-based vaccines.

PubMed: 39402031
DOI: 10.1038/s41467-024-53301-6

実験手法

ELECTRON MICROSCOPY (3 Å)