8Z1F

Cryo-EM structure of human ELAC2-tRNA

8Z1F の概要

• エントリーDOI: 10.2210/pdb8z1f/pdb
• EMDBエントリー: 39726
• 分子名称: Homo sapiens mitochondrion pre-tRNA-Tyr, Zinc phosphodiesterase ELAC protein 2, ZINC ION, ... (4 entities in total)
• 機能のキーワード: endonuclease zinc phosphodiesterase, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 120296.32

構造登録者

Liu, Z.M.,Xue, C.Y. (登録日: 2024-04-11, 公開日: 2024-12-18, 最終更新日: 2025-06-25)

主引用文献

Xue, C.,Tian, J.,Chen, Y.,Liu, Z.
Structural insights into human ELAC2 as a tRNA 3' processing enzyme.
Nucleic Acids Res., 52:13434-13446, 2024

PubMed Abstract: Human elaC ribonuclease Z 2 (ELAC2) removes the 3' trailer of precursor transfer ribonucleic acid (pre-tRNA). Mutations in ELAC2 are highly associated with the development of prostate cancer and hypertrophic cardiomyopathy. However, the catalytic mechanism of ELAC2 remains unclear. We determined the cryogenic electron microscopy structures of human ELAC2 in various states, including the apo, pre-tRNA-bound and tRNA-bound states, which enabled us to identify the structural basis for its binding to pre-tRNA and cleavage of the 3' trailer. Notably, conformational rearrangement of the C-terminal helix was related to feeding of the 3' trailer into the cleavage site, possibly explaining why its mutations are associated with disease. We further used biochemical assays to analyse the structural effects of disease-related mutations of human ELAC2. Collectively, our data provide a comprehensive structural basis for how ELAC2 recruits pre-tRNA via its flexible arm domain and guides the 3' trailer of pre-tRNA into the active centre for cleavage by its C-terminal helix.

PubMed: 39494506
DOI: 10.1093/nar/gkae1014

実験手法

ELECTRON MICROSCOPY (4.3 Å)