8Z0O

Cryo-EM structure of chitin synthase

8Z0O の概要

• エントリーDOI: 10.2210/pdb8z0o/pdb
• EMDBエントリー: 39709
• 分子名称: chitin synthase, URIDINE-5'-DIPHOSPHATE, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: chitin synthase, membrane-integrated glycosyltransferase, transferase
• 由来する生物種: Phytophthora sojae strain P6497
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 210090.85

構造登録者

Zhang, X.,Niu, S.,Li, P.,Bi, Y. (登録日: 2024-04-10, 公開日: 2024-12-04)

主引用文献

Niu, S.,Qi, L.,Zhang, X.,He, D.,Li, P.,Wang, H.,Bi, Y.
Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase.
Int J Mol Sci, 25:-, 2024

PubMed Abstract: Chitin, an extracellular polysaccharide, is synthesized by membrane-embedded chitin synthase (CHS) utilizing intracellular substrates. The mechanism of the translocation of synthesized chitin across the membrane to extracellular locations remains unresolved. We prove that the chitin synthase from (CHS) is a processive glycosyltransferase, which can rapidly produce and tightly bind with the highly polymerized chitin. We further demonstrate that CHS is a bifunctional enzyme, which is necessary and sufficient to translocate the synthesized chitin. CHS was purified and then reconstituted into proteoliposomes (PLs). The nascent chitin is generated and protected from chitinase degradation unless detergent solubilizes the PLs, showing that CHS translocates the newly produced chitin into the lumen of the PLs. We also attempted to resolve the CHS structure of the synthesized chitin-bound state, although it was not successful; the obtained high-resolution structure of the UDP/Mn-bound state could still assist in describing the characterization of the CHS's transmembrane channel. Consistently, we demonstrate that CHS is indispensable and capable of translocating chitin in a process that is tightly coupled to chitin synthesis.

PubMed: 39519219
DOI: 10.3390/ijms252111667

実験手法

ELECTRON MICROSCOPY (3.2 Å)