8YT0

Structure of CylI in complex with acyl-SNAC substrate

これはPDB形式変換不可エントリーです。

8YT0 の概要

• エントリーDOI: 10.2210/pdb8yt0/pdb
• 分子名称: CylI, ~{S}-(2-acetamidoethyl) (6~{S})-6-chloranyldecanethioate (3 entities in total)
• 機能のキーワード: cyanobacteria, cylindrocyclophane, type iii pks, transferase
• 由来する生物種: Cylindrospermum licheniforme UTEX B 2014
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87070.42

構造登録者

Wang, H.Q.,Xiang, Z. (登録日: 2024-03-24, 公開日: 2025-02-26)

主引用文献

Wang, H.Q.,Xiang, Z.
Structural insights into type III polyketide synthase CylI from cylindrocyclophane biosynthesis.
Protein Sci., 33:e5130-e5130, 2024

PubMed Abstract: Type III polyketide synthases (PKSs) catalyze the formation of a variety of polyketide natural products with remarkable structural diversity and biological activities. Despite significant progress in structural and mechanistic studies of type III PKSs in bacteria, fungi, and plants, research on type III PKSs in cyanobacteria is lacking. Here, we report structural and mechanistic insights into CylI, a type III PKS that catalyzes the formation of the alkylresorcinol intermediate in cylindrocyclophane biosynthesis. The crystal structure of apo-CylI reveals a distinct arrangement of structural elements that are proximal to the active site. We further solved the crystal structures of CylI in complexes with two substrate analogues at resolutions of 1.9 Å. The complex structures indicate that N259 is the key residue that determines the substrate preference of CylI. We also solved the crystal structure of CylI complexed with the alkylresorcinol product at a resolution of 2.0 Å. Structural analysis and mutagenesis experiments suggested that S170 functions as a key residue that determines cyclization specificity. On the basis of this result, a double mutant was engineered to completely switch the cyclization of CylI from aldol condensation to lactonization. This work elucidates the molecular basis of type III PKS in cyanobacteria and lays the foundation for engineering CylI-like enzymes to generate new products.

PubMed: 39302095
DOI: 10.1002/pro.5130

実験手法

X-RAY DIFFRACTION (1.9 Å)