8YS9

Crystal structure of Phosphatidylethanolamine N-methyltransferase from R. thermophilum complexed with DMPE and SAH

これはPDB形式変換不可エントリーです。

8YS9 の概要

• エントリーDOI: 10.2210/pdb8ys9/pdb
• 分子名称: Phosphatidylethanolamine N-methyltransferase/phosphatidyl-N-methylethanolamine N-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, [(2~{R})-3-[2-(dimethylamino)ethoxy-oxidanyl-phosphoryl]oxy-2-[(~{Z})-octadec-9-enoyl]oxy-propyl] (~{Z})-octadec-9-enoate, ... (7 entities in total)
• 機能のキーワード: phosphatidylethanolamine, phosphatidylcholine, bacterial phosphatidylcholine biosynthesis, peripheral membrane protein, sam-dependent methyltransferase, membrane protein, pmta, transferase
• 由来する生物種: Rubellimicrobium thermophilum DSM 16684
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50699.32

構造登録者

Kim, J.,Salsabila, S.D. (登録日: 2024-03-22, 公開日: 2024-10-16)

主引用文献

Salsabila, S.D.,Kim, J.
Structural insights into phosphatidylethanolamine N -methyltransferase PmtA mediating bacterial phosphatidylcholine synthesis.
Sci Adv, 10:eadr0122-eadr0122, 2024

PubMed Abstract: Phosphatidylethanolamine -methyltransferase (PmtA) catalyzes the biosynthesis of phosphatidylcholine (PC) from phosphatidylethanolamine (PE). Although PC is one of the major phospholipids constituting bilayer membranes in eukaryotes, certain bacterial species encode PmtA, a membrane-associated methyltransferase, to produce PC, which is correlated with cellular stress responses, adaptability to environmental changes, and symbiosis or virulence with eukaryotic hosts. Depending on the organism, multiple PmtAs may be required for producing monomethyl- and dimethyl-PE derivatives along with PC, whereas in organisms such as , a single enzyme is sufficient to direct all three methylation steps. In this study, we present the x-ray crystal structures of PmtA from in complex with dimethyl-PE and -adenosyl-l-homocysteine, as well as in its lipid-free form. Moreover, we demonstrate that the enzyme associates with the cellular membrane via electrostatic interactions facilitated by a group of critical basic residues and can successively methylate PE and its methylated derivatives, culminating in the production of PC.

PubMed: 39356767
DOI: 10.1126/sciadv.adr0122

実験手法

X-RAY DIFFRACTION (1.46 Å)