8YRC

Chlorinated YabJ from Staphylococcus aureus

8YRC の概要

• エントリーDOI: 10.2210/pdb8yrc/pdb
• 関連するPDBエントリー: 5YU2
• 分子名称: Translation initiation inhibitor homologue, OXYGEN ATOM, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: ribonuclease, stress-response protein, structural protein
• 由来する生物種: Staphylococcus aureus subsp. aureus Mu50
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 42903.60

構造登録者

Jeong, C.,Kim, H.J. (登録日: 2024-03-21, 公開日: 2024-04-24)

主引用文献

Jeong, C.,Kim, H.J.
YabJ from Staphylococcus aureus entraps chlorides within its pocket.
Biochem.Biophys.Res.Commun., 710:149892-149892, 2024

PubMed Abstract: Chlorination is a potent disinfectant against various microorganisms, including bacteria and viruses, by inducing protein modifications and functional changes. Chlorine, in the form of sodium hypochlorite, stands out as the predominant sanitizer choice due to its cost-effectiveness and powerful antimicrobial properties. Upon exposure to chlorination, proteins undergo modifications, with amino acids experiencing alterations through the attachment of chloride or oxygen atoms. These modifications lead to shifts in protein function and the modulation of downstream signaling pathways, ultimately resulting in a bactericidal effect. However, certain survival proteins, such as chaperones or transcription factors, aid organisms in overcoming harsh chlorination conditions. The expression of YabJ, a highly conserved protein from Staphylococcus aureus, is regulated by a stress-activated sigma factor called sigma B (σB). This research revealed that S. aureus YabJ maintains its structural integrity even under intense chlorination conditions and harbors sodium hypochlorite molecules within its surface pocket. Notably, the pocket of S. aureus YabJ is primarily composed of amino acids less susceptible to chlorination-induced damage, rendering it resistant to such effects. This study elucidates how S. aureus YabJ evades the detrimental effects of chlorination and highlights its role in sequestering sodium hypochlorite within its structure. Consequently, this process enhances resilience and facilitates adaptation to challenging environmental conditions.

PubMed: 38581951
DOI: 10.1016/j.bbrc.2024.149892

実験手法

X-RAY DIFFRACTION (1.7 Å)