8YOO
Cryo-EM structure of the human 80S ribosome with 100 um Tigecycline
This is a non-PDB format compatible entry.
Summary for 8YOO
| Entry DOI | 10.2210/pdb8yoo/pdb |
| EMDB information | 39455 |
| Descriptor | 28S rRNA, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (84 entities in total) |
| Functional Keywords | ribosome, tigecycline, antibiotic, eef2, serbp1 |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 81 |
| Total formula weight | 3842673.36 |
| Authors | |
| Primary citation | Li, X.,Wang, M.,Denk, T.,Buschauer, R.,Li, Y.,Beckmann, R.,Cheng, J. Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline. Nat Commun, 15:5481-5481, 2024 Cited by PubMed Abstract: Tigecycline is widely used for treating complicated bacterial infections for which there are no effective drugs. It inhibits bacterial protein translation by blocking the ribosomal A-site. However, even though it is also cytotoxic for human cells, the molecular mechanism of its inhibition remains unclear. Here, we present cryo-EM structures of tigecycline-bound human mitochondrial 55S, 39S, cytoplasmic 80S and yeast cytoplasmic 80S ribosomes. We find that at clinically relevant concentrations, tigecycline effectively targets human 55S mitoribosomes, potentially, by hindering A-site tRNA accommodation and by blocking the peptidyl transfer center. In contrast, tigecycline does not bind to human 80S ribosomes under physiological concentrations. However, at high tigecycline concentrations, in addition to blocking the A-site, both human and yeast 80S ribosomes bind tigecycline at another conserved binding site restricting the movement of the L1 stalk. In conclusion, the observed distinct binding properties of tigecycline may guide new pathways for drug design and therapy. PubMed: 38942792DOI: 10.1038/s41467-024-49797-7 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2 Å) |
Structure validation
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