8YOA

Crystal structure of endolytic transglycosylase MltG

8YOA の概要

• エントリーDOI: 10.2210/pdb8yoa/pdb
• 分子名称: Endolytic murein transglycosylase (2 entities in total)
• 機能のキーワード: mltg, lytic transglycosylase, peptidoglycan, membrane binding protein, membrane protein, lyase
• 由来する生物種: Mycobacteroides abscessus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37383.53

構造登録者

Lee, G.H.,Park, H.H. (登録日: 2024-03-13, 公開日: 2024-11-13)

主引用文献

Lee, G.H.,Kim, S.,Kim, D.Y.,Han, J.H.,Lee, S.Y.,Lee, J.H.,Lee, C.S.,Park, H.H.
Structure of MltG from Mycobacterium abscessus reveals structural plasticity between composed domains.
Iucrj, 11:903-909, 2024

PubMed Abstract: MltG, a membrane-bound lytic transglycosylase, has roles in terminating glycan polymerization in peptidoglycan and incorporating glycan chains into the cell wall, making it significant in bacterial cell-wall biosynthesis and remodeling. This study provides the first reported MltG structure from Mycobacterium abscessus (maMltG), a superbug that has high antibiotic resistance. Our structural and biochemical analyses revealed that MltG has a flexible peptidoglycan-binding domain and exists as a monomer in solution. Further, the putative active site of maMltG was disclosed using structural analysis and sequence comparison. Overall, this study contributes to our understanding of the transglycosylation reaction of the MltG family, aiding the design of next-generation antibiotics targeting M. abscessus.

PubMed: 39297240
DOI: 10.1107/S2052252524008443

実験手法

X-RAY DIFFRACTION (2.2 Å)