8YK6

Magnetic effects of thaumatin crystals; observation of the crystal growth of magneto-Archimedes levitation and magnetic orientation

8YK6 の概要

• エントリーDOI: 10.2210/pdb8yk6/pdb
• 分子名称: Thaumatin I, L(+)-TARTARIC ACID (3 entities in total)
• 機能のキーワード: sweet-tasting protein magneto-archimedes levitation, plant protein
• 由来する生物種: Thaumatococcus daniellii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22378.13

構造登録者

Nakabayashi, M.,Inaka, K.,Fujiwara, M.,Hagiwara, M.,Maki, S. (登録日: 2024-03-04, 公開日: 2025-02-26)

主引用文献

Maki, S.,Fujiwara, M.,Fujiwara, Y.,Nakabayashi, M.,Morimoto, S.,Tanaka, S.,Fujiwara, S.,Hagiwara, M.,Inaka, K.
Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation.
Plos One, 20:e0315335-e0315335, 2025

PubMed Abstract: We examined the magnetic effect of thaumatin crystals, which are a well known model of protein crystals but which have hardly been studied for that effect. We succeeded in crystallizing thaumatin by magnetic levitation based on the magneto-Archimedes effect by the addition of the paramagnetic substance gadolinium chloride. We also carried out a chronological observation of the levitation process in a superconducting magnet, and visualized the magnetic orientation of the crystals by applying a magnetic field along the horizontal direction. In another major result, we carried out a diffraction experiment and performed a structural analysis of the crystals. We noticed from the results that no electron density from the gadolinium ion could be observed in the crystals. This suggests the possibility that the paramagnetic substance of the aqueous precipitant solution helps only to promote the crystals' levitation, and has little effect on thaumatin crystallization.

PubMed: 39946396
DOI: 10.1371/journal.pone.0315335

実験手法

X-RAY DIFFRACTION (0.99 Å)