8YIM

Crystal Structure of Human Rab23 in complex with GMPPNP (1.35 Angstroms Resolution)

8YIM の概要

• エントリーDOI: 10.2210/pdb8yim/pdb
• 分子名称: Ras-related protein Rab-23, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: small gtpase rossmann fold, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19636.32

構造登録者

Chau, Y.Y.,Liang, H.,Aik, W.S. (登録日: 2024-02-29, 公開日: 2024-12-04, 最終更新日: 2025-01-08)

主引用文献

Chau, Y.Y.,Liang, H.,Tung, W.L.,Hor, C.H.H.,Aik, W.S.
Structural basis for Rab23 activation and a loss-of-function mutation in Carpenter syndrome.
J.Biol.Chem., 301:108036-108036, 2024

PubMed Abstract: Rab23 is a member of the Rab family of small GTPases. It plays crucial roles in Hedgehog signaling, ciliary transport, and embryonic development. As a small GTPase, Rab23 cycles between the GDP-bound inactivated state and the GTP-bound activated state. Mutations in Rab23 are directly implicated in Carpenter syndrome, a development disorder characterized by deformed skulls, abnormal fingers or toes, and intellectual disabilities. Several clinical point mutations, for example, M12K, C85R, and Y79del, have been found to occur within the GTPase domain. However, the mechanisms of activation of Rab23 and pathogenesis of its clinical mutants are still unclear with limited structural information. So far, there are only two reported crystal structures of mouse Rab23 in complex with GDP. Here, we determined high-resolution crystal structures of human Rab23 and the human Rab23 Y79del clinical mutant, in complex with GDP and GMPPNP, a nonhydrolysable GTP analog, respectively. Supported by in vitro biochemical and functional analyses, we demonstrated that the Y79 deletion mutant exhibited structural distortions in the switch II region relative to that of the WT. The structural changes potentially disrupted the binding of Rab23 Y79del to its interacting partners, thus leading to a loss-of-function and the development of Carpenter syndrome.

PubMed: 39615683
DOI: 10.1016/j.jbc.2024.108036

実験手法

X-RAY DIFFRACTION (1.35 Å)