8YFE

Cryo EM structure of Komagataella phaffii Rat1-Rai1 complex

8YFE の概要

• エントリーDOI: 10.2210/pdb8yfe/pdb
• EMDBエントリー: 39221
• 分子名称: 5'-3' exoribonuclease, Decapping nuclease (2 entities in total)
• 機能のキーワード: transcription termination, rna polymerase ii, transcription
• 由来する生物種: Komagataella phaffii; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 319820.91

構造登録者

Yanagisawa, T.,Murayama, Y.,Ehara, H.,Sekine, S.I. (登録日: 2024-02-24, 公開日: 2024-09-25, 最終更新日: 2025-06-18)

主引用文献

Yanagisawa, T.,Murayama, Y.,Ehara, H.,Goto, M.,Aoki, M.,Sekine, S.I.
Structural basis of eukaryotic transcription termination by the Rat1 exonuclease complex.
Nat Commun, 15:7854-7854, 2024

PubMed Abstract: The 5´-3´ exoribonuclease Rat1/Xrn2 is responsible for the termination of eukaryotic mRNA transcription by RNAPII. Rat1 forms a complex with its partner proteins, Rai1 and Rtt103, and acts as a "torpedo" to bind transcribing RNAPII and dissociate DNA/RNA from it. Here we report the cryo-electron microscopy structures of the Rat1-Rai1-Rtt103 complex and three Rat1-Rai1-associated RNAPII complexes (type-1, type-1b, and type-2) from the yeast, Komagataella phaffii. The Rat1-Rai1-Rtt103 structure revealed that Rat1 and Rai1 form a heterotetramer with a single Rtt103 bound between two Rai1 molecules. In the type-1 complex, Rat1-Rai1 forms a heterodimer and binds to the RNA exit site of RNAPII to extract RNA into the Rat1 exonuclease active site. This interaction changes the RNA path in favor of termination (the "pre-termination" state). The type-1b and type-2 complexes have no bound DNA/RNA, likely representing the "post-termination" states. These structures illustrate the termination mechanism of eukaryotic mRNA transcription.

PubMed: 39245712
DOI: 10.1038/s41467-024-52157-0

実験手法

ELECTRON MICROSCOPY (3.47 Å)