8YEK
Cryo-EM structure of the channelrhodopsin GtCCR2
8YEK の概要
エントリーDOI | 10.2210/pdb8yek/pdb |
EMDBエントリー | 39198 |
分子名称 | GtCCR2, RETINAL, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total) |
機能のキーワード | microbial rhodopsin, membrane protein |
由来する生物種 | Guillardia theta CCMP2712 |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 54689.95 |
構造登録者 | Tanaka, T.,Iida, W.,Sano, F.K.,Oda, K.,Shihoya, W.,Nureki, O. (登録日: 2024-02-22, 公開日: 2024-09-04, 最終更新日: 2024-11-06) |
主引用文献 | Tanaka, T.,Hososhima, S.,Yamashita, Y.,Sugimoto, T.,Nakamura, T.,Shigemura, S.,Iida, W.,Sano, F.K.,Oda, K.,Uchihashi, T.,Katayama, K.,Furutani, Y.,Tsunoda, S.P.,Shihoya, W.,Kandori, H.,Nureki, O. The high-light-sensitivity mechanism and optogenetic properties of the bacteriorhodopsin-like channelrhodopsin GtCCR4. Mol.Cell, 84:3530-3544.e6, 2024 Cited by PubMed Abstract: Channelrhodopsins are microbial light-gated ion channels that can control the firing of neurons in response to light. Among several cation channelrhodopsins identified in Guillardia theta (GtCCRs), GtCCR4 has higher light sensitivity than typical channelrhodopsins. Furthermore, GtCCR4 shows superior properties as an optogenetic tool, such as minimal desensitization. Our structural analyses of GtCCR2 and GtCCR4 revealed that GtCCR4 has an outwardly bent transmembrane helix, resembling the conformation of activated G-protein-coupled receptors. Spectroscopic and electrophysiological comparisons suggested that this helix bend in GtCCR4 omits channel recovery time and contributes to high light sensitivity. An electrophysiological comparison of GtCCR4 and the well-characterized optogenetic tool ChRmine demonstrated that GtCCR4 has superior current continuity and action-potential spike generation with less invasiveness in neurons. We also identified highly active mutants of GtCCR4. These results shed light on the diverse structures and dynamics of microbial rhodopsins and demonstrate the strong optogenetic potential of GtCCR4. PubMed: 39232582DOI: 10.1016/j.molcel.2024.08.016 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (2.73 Å) |
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