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8YEK

Cryo-EM structure of the channelrhodopsin GtCCR2

8YEK の概要
エントリーDOI10.2210/pdb8yek/pdb
EMDBエントリー39198
分子名称GtCCR2, RETINAL, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
機能のキーワードmicrobial rhodopsin, membrane protein
由来する生物種Guillardia theta CCMP2712
タンパク質・核酸の鎖数1
化学式量合計54689.95
構造登録者
Tanaka, T.,Iida, W.,Sano, F.K.,Oda, K.,Shihoya, W.,Nureki, O. (登録日: 2024-02-22, 公開日: 2024-09-04, 最終更新日: 2024-11-06)
主引用文献Tanaka, T.,Hososhima, S.,Yamashita, Y.,Sugimoto, T.,Nakamura, T.,Shigemura, S.,Iida, W.,Sano, F.K.,Oda, K.,Uchihashi, T.,Katayama, K.,Furutani, Y.,Tsunoda, S.P.,Shihoya, W.,Kandori, H.,Nureki, O.
The high-light-sensitivity mechanism and optogenetic properties of the bacteriorhodopsin-like channelrhodopsin GtCCR4.
Mol.Cell, 84:3530-3544.e6, 2024
Cited by
PubMed Abstract: Channelrhodopsins are microbial light-gated ion channels that can control the firing of neurons in response to light. Among several cation channelrhodopsins identified in Guillardia theta (GtCCRs), GtCCR4 has higher light sensitivity than typical channelrhodopsins. Furthermore, GtCCR4 shows superior properties as an optogenetic tool, such as minimal desensitization. Our structural analyses of GtCCR2 and GtCCR4 revealed that GtCCR4 has an outwardly bent transmembrane helix, resembling the conformation of activated G-protein-coupled receptors. Spectroscopic and electrophysiological comparisons suggested that this helix bend in GtCCR4 omits channel recovery time and contributes to high light sensitivity. An electrophysiological comparison of GtCCR4 and the well-characterized optogenetic tool ChRmine demonstrated that GtCCR4 has superior current continuity and action-potential spike generation with less invasiveness in neurons. We also identified highly active mutants of GtCCR4. These results shed light on the diverse structures and dynamics of microbial rhodopsins and demonstrate the strong optogenetic potential of GtCCR4.
PubMed: 39232582
DOI: 10.1016/j.molcel.2024.08.016
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (2.73 Å)
構造検証レポート
Validation report summary of 8yek
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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