8YBM

Crystal structure of nanobody SEB-Nb6 bound to staphylococcal enterotoxin B (SEB)

8YBM の概要

• エントリーDOI: 10.2210/pdb8ybm/pdb
• 分子名称: Enterotoxin type B, nanobody SEB-Nb6 (3 entities in total)
• 機能のキーワード: antibody, nanobody, toxin, staphylococcal enterotoxin b (seb), antitoxin
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 84935.12

構造登録者

Ding, Y.,Zong, X.,Liu, R.,Liu, P. (登録日: 2024-02-15, 公開日: 2024-12-25, 最終更新日: 2025-01-01)

主引用文献

Zong, X.,Liu, P.,Wang, Z.,Zhu, H.,Zhong, C.,Zhong, P.,Jiang, H.,Liu, J.,Ma, Z.,Liu, X.,Liu, R.,Ding, Y.
Structural insights into the binding of nanobodies to the Staphylococcal enterotoxin B.
Int.J.Biol.Macromol., 276:133957-133957, 2024

PubMed Abstract: Staphylococcal Enterotoxin Type B (SEB), produced by Staphylococcus aureus bacteria, is notorious for inducing severe food poisoning and toxic shock syndrome. While nanobody-based treatments hold promises for combating SEB-induced diseases, the lack of structural information between SEB and nanobodies has hindered the development of nanobody-based therapeutics. Here, we present crystal structures of SEB-Nb3, SEB-Nb6, SEB-Nb8, SEB-Nb11, and SEB-Nb20 at resolutions ranging from 1.59 Å to 2.33 Å. Crystallographic analysis revealed that Nb3, Nb8, Nb11, and Nb20 bind to SEB at the T-cell receptor (TCR) interface, while Nb6 binds at the major histocompatibility complex (MHC) interface, suggesting their potential to inhibit SEB function by disrupting interactions with TCR or MHC molecules. Molecular biological analyses confirmed the thermodynamic and kinetic parameters of Nb3, Nb5, Nb6, Nb8, Nb11, Nb15, Nb18, and Nb20 to SEB. The competitive inhibition was further confirmed by cell-based experiments demonstrating nanobody neutralization. These findings elucidate the structural basis for developing specific nanobodies to neutralize SEB threats, providing crucial insights into the underlying mechanisms and offering significant assistance for further optimization towards future therapeutic strategies.

PubMed: 39029852
DOI: 10.1016/j.ijbiomac.2024.133957

実験手法

X-RAY DIFFRACTION (2.04 Å)