8Y9J

Structure of the Ebola virus nucleocapsid subunit

8Y9J の概要

• エントリーDOI: 10.2210/pdb8y9j/pdb
• EMDBエントリー: 39081
• 分子名称: Nucleoprotein, Membrane-associated protein VP24, RNA (12-MER) (3 entities in total)
• 機能のキーワード: complex, viral protein, viral protein-rna complex, viral protein/rna
• 由来する生物種: Zaire ebolavirus; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 226905.65

構造登録者

Fujita-Fujiharu, Y.,Hu, S.,Hirabayashi, A.,Takamatsu, Y.,Ng, Y.,Houri, K.,Muramoto, Y.,Nakano, M.,Sugita, Y.,Noda, T. (登録日: 2024-02-06, 公開日: 2025-01-29, 最終更新日: 2025-07-02)

主引用文献

Fujita-Fujiharu, Y.,Hu, S.,Hirabayashi, A.,Takamatsu, Y.,Ng, Y.N.,Houri, K.,Muramoto, Y.,Nakano, M.,Sugita, Y.,Noda, T.
Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24.
Nat Commun, 16:2171-2171, 2025

PubMed Abstract: The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain a helical nucleocapsid responsible for genome transcription, replication, and packaging into progeny virions. The nucleocapsid consists of a helical nucleoprotein (NP)-viral genomic RNA complex forming the core structure, to which VP24 and VP35 bind externally. Two NPs, each paired with a VP24 molecule, constitute a repeating unit. However, the detailed nucleocapsid structure remains unclear. Here, we determine the nucleocapsid-like structure within virus-like particles at 4.6 Å resolution using single-particle cryo-electron microscopy. Mutational analysis identifies specific interactions between the two NPs and two VP24s and demonstrates that each of the two VP24s in different orientations distinctively regulates nucleocapsid assembly, viral RNA synthesis, intracellular transport of the nucleocapsid, and infectious virion production. Our findings highlight the sophisticated mechanisms underlying the assembly and functional regulation of the nucleocapsid and provide insights into antiviral development.

PubMed: 40064872
DOI: 10.1038/s41467-025-57236-4

実験手法

ELECTRON MICROSCOPY (4.6 Å)