8XYJ

Structure of y+LAT1 bound with Lys

8XYJ の概要

• エントリーDOI: 10.2210/pdb8xyj/pdb
• EMDBエントリー: 38775
• 分子名称: Y+L amino acid transporter 1, LYSINE (2 entities in total)
• 機能のキーワード: complex, membrane protein, amino acid
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58110.33

構造登録者

Yan, R.H.,Dai, L.,Zhang, T. (登録日: 2024-01-19, 公開日: 2025-01-22, 最終更新日: 2025-04-02)

主引用文献

Dai, L.,Zeng, Q.,Zhang, T.,Zhang, Y.,Shi, Y.,Li, Y.,Xu, K.,Huang, J.,Wang, Z.,Zhou, Q.,Yan, R.
Structural basis for the substrate recognition and transport mechanism of the human y + LAT1-4F2hc transporter complex.
Sci Adv, 11:eadq0558-eadq0558, 2025

PubMed Abstract: Heteromeric amino acid transporters (HATs), including yLAT1-4F2hc complex, are responsible for transporting amino acids across membranes, and mutations in yLAT1 cause lysinuric protein intolerance (LPI), a hereditary disorder characterized by defective cationic amino acid transport. The relationship between LPI and specific mutations in yLAT1 has yet to be fully understood. In this study, we characterized the function of yLAT1-4F2hc complex in mammalian cells and determined the cryo-EM structures of the human yLAT1-4F2hc complex in two distinct conformations: the apo state in an inward-open conformation and the native substrate-bound state in an outward-open conformation. Structural analysis suggests that Asp in yLAT1 plays a crucial role in coordination with sodium ion and substrate selectivity. Molecular dynamic (MD) simulations further revealed the different transport mechanism of cationic amino acids and neutral amino acids. These results provide important insights into the mechanisms of the substrate binding and working cycle of HATs.

PubMed: 40106545
DOI: 10.1126/sciadv.adq0558

実験手法

ELECTRON MICROSCOPY (3.33 Å)