8XSQ

Pseudomonas aeruginosa Histidinol dehydrogenase native structure without Zn+

8XSQ の概要

• エントリーDOI: 10.2210/pdb8xsq/pdb
• 分子名称: Histidinol dehydrogenase (2 entities in total)
• 機能のキーワード: dehydrogenase, oxidoreductase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94458.62

構造登録者

Choudhury, G.B.,Datta, S. (登録日: 2024-01-09, 公開日: 2025-01-15, 最終更新日: 2025-12-24)

主引用文献

Basu Choudhury, G.,Chatterjee, R.,Saha, A.,Sarkar, D.J.,Das, B.K.,Datta, S.
Crystal structure-guided revelation of metal ion-dependent functional ambiguity in Pseudomonas aeruginosa histidinol dehydrogenase.
Febs J., 292:6432-6453, 2025

PubMed Abstract: Histidinol dehydrogenase (HisD) is an enzyme that catalyzes the final step in histidine biosynthesis, converting l-histidinol to l-histidine, and plays a crucial role in bacterial metabolism. In this study, we investigated the ambiguity in catalytic mechanisms of the HisD enzyme in Pseudomonas aeruginosa using biochemical and structural approaches, particularly through X-ray crystallography. The primary objective of this research was to explore the structural and functional variability of PaHisD and provide knowledge for potential therapeutic developments in this organism. Our findings reveal significant structural alterations in the enzyme as we identified a new substrate-binding pocket due to structural rearrangements. We also confirmed the presence of an additional metal ion (Zn), contributing to its catalytic ambiguity. Given its relevance in molecular drug targeting, we examined how the differences in NAD and substrate binding could impact the efficacy of existing inhibitors. Computational studies further evaluated the variability in inhibitor binding, providing new insights for designing more effective therapeutic agents targeting PaHisD.

PubMed: 40729526
DOI: 10.1111/febs.70209

実験手法

X-RAY DIFFRACTION (1.75 Å)