8XSE

SARS-CoV-2 RBD + IMCAS-123 + IMCAS-72 Fab

8XSE の概要

• エントリーDOI: 10.2210/pdb8xse/pdb
• EMDBエントリー: 38617
• 分子名称: Spike protein S1, IMCAS-123 H chain, IMCAS-123 L chain, ... (6 entities in total)
• 機能のキーワード: sars-cov-2, broadly neutralizing antibodies, virus, viral protein/immune system, viral protein-immune system complex
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 118404.88

構造登録者

Tong, Z.,Cui, Y.,Xie, Y.,Tong, J.,Gao, G.F.,Qi, J. (登録日: 2024-01-09, 公開日: 2024-07-17, 最終更新日: 2025-07-23)

主引用文献

Tong, Z.,Tong, J.,Lei, W.,Xie, Y.,Cui, Y.,Jia, G.,Li, S.,Zhang, Z.,Cheng, Z.,Xing, X.,Ma, H.,Deng, L.,Zhang, R.,Zhao, X.,Liu, K.,Wang, Q.,Qi, J.,Huang, H.,Song, R.,Su, Z.,Wu, G.,Lou, J.,Gao, G.F.
Deciphering a reliable synergistic bispecific strategy of rescuing antibodies for SARS-CoV-2 escape variants, including BA.2.86, EG.5.1, and JN.1.
Cell Rep, 43:114338-114338, 2024

PubMed Abstract: The game between therapeutic monoclonal antibodies (mAbs) and continuously emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has favored the virus, as most therapeutic mAbs have been evaded. Addressing this challenge, we systematically explored a reproducible bispecific antibody (bsAb)-dependent synergistic effect in this study. It could effectively restore the neutralizing activity of the bsAb when any of its single mAbs is escaped by variants. This synergy is primarily attributed to the binding angle of receptor-binding domain (RBD)-5, facilitating inter-spike cross-linking and promoting cryptic epitope exposure that classical antibody cocktails cannot achieve. Furthermore, RBD-5 with RBD-2, RBD-6, and RBD-7, alongside RBD-8, also exhibit significantly enhanced effects. This study not only shifts the paradigm in understanding antibody interactions but paves the way for developing more effective therapeutic antibodies against rapidly mutating SARS-CoV-2, with Dia-19 already showing promise against emerging variants like BA.2.86, EG.5.1, and JN.1.

PubMed: 38850530
DOI: 10.1016/j.celrep.2024.114338

実験手法

ELECTRON MICROSCOPY (2.5 Å)