8XOU

Prohead portal vertex of bacteriophage lambda

これはPDB形式変換不可エントリーです。

8XOU の概要

• エントリーDOI: 10.2210/pdb8xou/pdb
• EMDBエントリー: 38540 38541
• 分子名称: Portal protein B, Major capsid protein (2 entities in total)
• 機能のキーワード: caudovirales, siphoviridae, portal vertex, portal, capsid, connector/neck, tail, delivery device, b-dna, phage lambda, viral protein
• 由来する生物種: Escherichia phage Lambda; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 1861230.11

構造登録者

Wang, J.W.,Gu, Z.W. (登録日: 2024-01-02, 公開日: 2024-04-10, 最終更新日: 2024-11-27)

主引用文献

Gu, Z.,Wu, K.,Wang, J.
Structural morphing in the viral portal vertex of bacteriophage lambda.
J.Virol., 98:e0006824-e0006824, 2024

PubMed Abstract: The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA packaging is complete, additional proteins are assembled onto the portal to form the connector complex, which is crucial as it bridges the mature head and tail. In this study, we report high-resolution cryo-electron microscopy (cryo-EM) structures of the portal vertex from bacteriophage lambda in both its prohead and mature virion states. Comparison of these structures shows that during head maturation, in addition to capsid expansion, the portal protein undergoes conformational changes to establish interactions with the connector proteins. Additionally, the independently assembled tail undergoes morphological alterations at its proximal end, facilitating its connection to the head-tail joining protein and resulting in the formation of a stable portal-connector-tail complex. The B-DNA molecule spirally glides through the tube, interacting with the nozzle blade region of the middle-ring connector protein. These insights elucidate a mechanism for portal maturation and DNA translocation within the phage lambda system.

PubMed: 38661364
DOI: 10.1128/jvi.00068-24

実験手法

ELECTRON MICROSCOPY (5.58 Å)