8XOD

ThDP-dependent HKA synthase

これはPDB形式変換不可エントリーです。

8XOD の概要

• エントリーDOI: 10.2210/pdb8xod/pdb
• 分子名称: BbmA-G484F complex with CBOA, ADENOSINE-5'-DIPHOSPHATE, 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(~{R})-cyclobutyl(oxidanyl)methyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate, ... (6 entities in total)
• 機能のキーワード: thdp-dependent hka synthase, mutant of bbma, biosynthetic protein
• 由来する生物種: Brevibacillus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 253638.50

構造登録者

Yu, J.H.,Liu, T. (登録日: 2024-01-01, 公開日: 2025-01-01, 最終更新日: 2025-07-16)

主引用文献

Liu, T.,Wang, G.,Yu, J.,Li, M.,Peng, T.,Wang, J.,Li, H.,Su, X.D.,Jiang, C.,Ye, M.,Yang, D.,Ma, M.
Structural insights into two thiamine diphosphate-dependent enzymes and their synthetic applications in carbon-carbon linkage reactions.
Nat.Chem., 17:1107-1118, 2025

PubMed Abstract: The α-hydroxy-β-keto acid synthases are thiamine diphosphate-dependent enzymes catalysing carbon-carbon linkage reactions in the biosynthesis of primary metabolites and various secondary metabolites. However, the substrate selectivity and catalytic stereoselectivity of α-hydroxy-β-keto acid synthases are poorly understood, greatly hindering their synthetic application in generating diverse carbon frameworks. We here report the discovery of two new α-hydroxy-β-keto acid synthases CsmA and BbmA, which show different substrate selectivities in catalysing carbon-carbon coupling reactions between two β-keto acids. Four crystal structures of CsmA or BbmA complexed with thiamine diphosphate and their substrates were determined, clearly revealing their structural bases of substrate selectivity and catalytic stereoselectivity. Substrate scope expansion enables us to generate 120 α-hydroxy-β-keto acids together with 240 NaBH-reduction products. Furthermore, we applied CsmA and BbmA into enzymatic total synthesis, generating 36 γ-butyrolactone-containing furanolides. These results provide new structural insights into the catalyses of α-hydroxy-β-keto acid synthases and highlight their great potential in carboligation catalysis and synthetic applications.

PubMed: 40369129
DOI: 10.1038/s41557-025-01822-y

実験手法

X-RAY DIFFRACTION (2.75 Å)