8XMB の概要
| エントリーDOI | 10.2210/pdb8xmb/pdb |
| EMDBエントリー | 38470 |
| 分子名称 | DNA-directed RNA polymerase IV subunit 1, DNA-directed RNA polymerases II, IV and V subunit 10, DNA-directed RNA polymerases II, IV and V subunit 11, ... (19 entities in total) |
| 機能のキーワード | transcription, elongation, pol iv, rna polymerase, transcription/dna/rna, transcription-dna-rna complex |
| 由来する生物種 | Arabidopsis thaliana (thale cress) 詳細 |
| タンパク質・核酸の鎖数 | 16 |
| 化学式量合計 | 629119.18 |
| 構造登録者 | |
| 主引用文献 | Fang, C.,Huang, K.,Wu, X.,Zhang, H.,Gu, Z.,Wang, J.,Zhang, Y. Transcription elongation of the plant RNA polymerase IV is prone to backtracking. Sci Adv, 10:eadq3087-eadq3087, 2024 Cited by PubMed Abstract: RNA polymerase IV (Pol IV) forms a complex with RNA-directed RNA polymerase 2 (RDR2) to produce double-stranded RNA (dsRNA) precursors essential for plant gene silencing. In the "backtracking-triggered RNA channeling" model, Pol IV backtracks and delivers its transcript's 3' terminus to RDR2, which synthesizes dsRNA. However, the mechanisms underlying Pol IV backtracking and RNA protection from cleavage are unclear. Here, we determined cryo-electron microscopy structures of Pol IV elongation complexes at four states of its nucleotide addition cycle (NAC): posttranslocation, guanosine triphosphate-bound, pretranslocation, and backtracked states. The structures reveal that Pol IV maintains an open DNA cleft and kinked bridge helix in all NAC states, loosely interacts with the nucleoside triphosphate substrate, and barely contacts proximal backtracked nucleotides. Biochemical data indicate that Pol IV is inefficient in forward translocation and RNA cleavage. These findings suggest that Pol IV transcription elongation is prone to backtracking and incapable of RNA hydrolysis, ensuring efficient dsRNA production by Pol IV-RDR2. PubMed: 39178250DOI: 10.1126/sciadv.adq3087 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.4 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
