8XIT

Cryo-EM structure of sheep VMAT2 dimer in an atypical fold

8XIT の概要

• エントリーDOI: 10.2210/pdb8xit/pdb
• EMDBエントリー: 38389
• 分子名称: OaVMAT2-BRIL (1 entity in total)
• 機能のキーワード: vesicular monoamine transporter, slc18a2, mfs, apo, conformation, transport protein
• 由来する生物種: Ovis aries
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 137185.38

構造登録者

Lyu, Y.,Fu, C.,Ma, H.,Sun, Z.,Su, Z.,Zhou, X. (登録日: 2023-12-20, 公開日: 2024-08-14)

主引用文献

Lyu, Y.,Fu, C.,Ma, H.,Su, Z.,Sun, Z.,Zhou, X.
Engineering of a mammalian VMAT2 for cryo-EM analysis results in non-canonical protein folding.
Nat Commun, 15:6511-6511, 2024

PubMed Abstract: Vesicular monoamine transporter 2 (VMAT2) belongs to the major facilitator superfamily (MFS), and mediates cytoplasmic monoamine packaging into presynaptic vesicles. Here, we present two cryo-EM structures of VMAT2, with a frog VMAT2 adopting a canonical MFS fold and an engineered sheep VMAT2 adopting a non-canonical fold. Both VMAT2 proteins mediate uptake of a selective fluorescent VMAT2 substrate into cells. Molecular docking, substrate binding and transport analysis reveal potential substrate binding mechanism in VMAT2. Meanwhile, caution is advised when interpreting engineered membrane protein structures.

PubMed: 39095428
DOI: 10.1038/s41467-024-50934-5

実験手法

ELECTRON MICROSCOPY (3.2 Å)