8XAI

Crystal structure of Protease CPAVM1 in Bacillus subtilis LjM2

Summary for 8XAI

• Entry DOI: 10.2210/pdb8xai/pdb
• Descriptor: Lipoprotein (2 entities in total)
• Functional Keywords: influenza a virus, sars-cov-2, bacillus subtilis ljm2, protease cpavm1, antiviral protein
• Biological source: Bacillus subtilis
• Total number of polymer chains: 4
• Total formula weight: 100136.52

Authors

Zhang, J.,Wang, C.Y. (deposition date: 2023-12-04, release date: 2024-06-19, Last modification date: 2024-07-10)

Primary citation

Li, J.,Cui, H.,Yao, Y.,Niu, J.,Zhang, J.,Zheng, X.,Cui, M.,Liu, J.,Cheng, T.,Gao, Y.,Guo, Q.,Yu, S.,Wang, L.,Huang, Z.,Huang, J.,Zhang, K.,Wang, C.,Meng, G.
Anti-influenza activity of CPAVM1 protease secreted by Bacillus subtilis LjM2.
Antiviral Res., 228:105919-105919, 2024

PubMed Abstract: Bacillus spp. has been considered a promising source for identifying new antimicrobial substances, including anti-viral candidates. Here, we successfully isolated a number of bacteria strains from aged dry citrus peel (Chenpi). Of note, the culture supernatant of a new isolate named Bacillus subtilis LjM2 demonstrated strong inhibition of influenza A virus (IAV) infection in multiple experimental systems in vitro and in vivo. In addition, the anti-viral effect of LjM2 was attributed to its direct lysis of viral particles. Further analysis showed that a protease which we named CPAVM1 isolated from the culture supernatant of LjM2 was the key component responsible for its anti-viral function. Importantly, the therapeutic effect of CPAVM1 was still significant when applied 12 hours after IAV infection of experimental mice. Moreover, we found that the CPAVM1 protease cleaved multiple IAV proteins via targeting basic amino acid Arg or Lys. Furthermore, this study reveals the molecular structure and catalytic mechanism of CPAVM1 protease. During catalysis, Tyr75, Tyr77, and Tyr102 are important active sites. Therefore, the present work identified a special protease CPAVM1 secreted by a new strain of Bacillus subtilis LjM2 against influenza A virus infection via direct cleavage of critical viral proteins, thus facilitates future biotechnological applications of Bacillus subtilis LjM2 and the protease CPAVM1.

PubMed: 38851592
DOI: 10.1016/j.antiviral.2024.105919

Experimental method

X-RAY DIFFRACTION (1.83 Å)