8X82

The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster

8X82 の概要

• エントリーDOI: 10.2210/pdb8x82/pdb
• EMDBエントリー: 38133
• 分子名称: Gustatory receptor for sugar taste 43a (1 entity in total)
• 機能のキーワード: gustatory receptor, ligand-gated ion channel, gr43a, membrane protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 197069.25

構造登録者

Ma, D.,Guo, J. (登録日: 2023-11-27, 公開日: 2024-02-07, 最終更新日: 2024-03-06)

主引用文献

Ma, D.,Hu, M.,Yang, X.,Liu, Q.,Ye, F.,Cai, W.,Wang, Y.,Xu, X.,Chang, S.,Wang, R.,Yang, W.,Ye, S.,Su, N.,Fan, M.,Xu, H.,Guo, J.
Structural basis for sugar perception by Drosophila gustatory receptors.
Science, 383:eadj2609-eadj2609, 2024

PubMed Abstract: Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.

PubMed: 38305684
DOI: 10.1126/science.adj2609

実験手法

ELECTRON MICROSCOPY (2.8 Å)