8X7I

Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by intein-based E2-Ub-NCP conjugation strategy

Summary for 8X7I

• Entry DOI: 10.2210/pdb8x7i/pdb
• EMDB information: 38099
• Descriptor: Histone H3.2, Ubiquitin, ZINC ION, ... (11 entities in total)
• Functional Keywords: rnf168, nucleosome, h2ak13/15 ubiquitination, nuclear protein, nuclear protein-dna complex, nuclear protein/dna
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 13
• Total formula weight: 216262.02

Authors

Ai, H.S.,Tong, Z.B.,Deng, Z.H.,Pan, M.,Liu, L. (deposition date: 2023-11-24, release date: 2024-08-07)

• Primary citation: Ai, H.,Tong, Z.,Deng, Z.,Shi, Q.,Tao, S.,Liang, J.,Sun, M.,Wu, X.,Zheng, Q.,Liang, L.,Li, J.B.,Gao, S.,Tian, C.,Liu, L.,Pan, M.; Capturing Snapshots of Nucleosomal H2A K13/K15 Ubiquitination Mediated by the Monomeric E3 Ligase RNF168; Biorxiv, 2024; DOI: 10.1101/2024.01.02.573964

Experimental method

ELECTRON MICROSCOPY (3.27 Å)