8X7D

Crystal structure of OsHSL1 L204F/F298L/I335F

8X7D の概要

• エントリーDOI: 10.2210/pdb8x7d/pdb
• 分子名称: HPPD Inhibitor Sensitive 1-like 1 protein, COBALT (II) ION, 2-OXOGLUTARIC ACID, ... (4 entities in total)
• 機能のキーワード: complex, substrate, oxidoreductase
• 由来する生物種: Oryza sativa (Asian cultivated rice)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80736.61

構造登録者

Lin, H.-Y.,Dong, J.,Yang, G.-F. (登録日: 2023-11-23, 公開日: 2024-10-02)

主引用文献

Dong, J.,Yu, X.H.,Dong, J.,Wang, G.H.,Wang, X.L.,Wang, D.W.,Yan, Y.C.,Xiao, H.,Ye, B.Q.,Lin, H.Y.,Yang, G.F.
An artificially evolved gene for herbicide-resistant rice breeding.
Proc.Natl.Acad.Sci.USA, 121:e2407285121-e2407285121, 2024

PubMed Abstract: Discovering and engineering herbicide-resistant genes is a crucial challenge in crop breeding. This study focuses on the 4-hydroxyphenylpyruvate dioxygenase Inhibitor Sensitive 1-Like (HSL) protein, prevalent in higher plants and exhibiting weak catalytic activity against many β-triketone herbicides (β-THs). The crystal structures of maize HSL1A complexed with β-THs were elucidated, identifying four essential herbicide-binding residues and explaining the weak activity of HSL1A against the herbicides. Utilizing an artificial evolution approach, we developed a series of rice HSL1 mutants targeting the four residues. Then, these mutants were systematically evaluated, identifying the M10 variant as the most effective in modifying β-THs. The initial active conformation of substrate binding in HSL1 was also revealed from these mutants. Furthermore, overexpression of M10 in rice significantly enhanced resistance to β-THs, resulting in a notable 32-fold increase in resistance to methyl-benquitrione. In conclusion, the artificially evolved M10 gene shows great potential for the development of herbicide-resistant crops.

PubMed: 39133859
DOI: 10.1073/pnas.2407285121

実験手法

X-RAY DIFFRACTION (2.2 Å)