8WWF

Crystal structure of (R)-DHPS dehydrogenase HpsO from Ruegeria pomeroyi DSS-3

8WWF の概要

• エントリーDOI: 10.2210/pdb8wwf/pdb
• 分子名称: (R)-DHPS dehydrogenase HpsO (2 entities in total)
• 機能のキーワード: nad-dependent, oxidoreductase
• 由来する生物種: Ruegeria pomeroyi DSS-3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 105851.02

構造登録者

Liu, L.,Tang, K. (登録日: 2023-10-25, 公開日: 2024-09-04, 最終更新日: 2024-09-11)

主引用文献

Liu, L.,Gao, X.,Dong, C.,Wang, H.,Chen, X.,Ma, X.,Liu, S.,Chen, Q.,Lin, D.,Jiao, N.,Tang, K.
Enantioselective transformation of phytoplankton-derived dihydroxypropanesulfonate by marine bacteria.
Isme J, 18:-, 2024

PubMed Abstract: Chirality, a fundamental property of matter, is often overlooked in the studies of marine organic matter cycles. Dihydroxypropanesulfonate (DHPS), a globally abundant organosulfur compound, serves as an ecologically important currency for nutrient and energy transfer from phytoplankton to bacteria in the ocean. However, the chirality of DHPS in nature and its transformation remain unclear. Here, we developed a novel approach using chiral phosphorus-reagent labeling to separate DHPS enantiomers. Our findings demonstrated that at least one enantiomer of DHPS is present in marine diatoms and coccolithophores, and that both enantiomers are widespread in marine environments. A novel chiral-selective DHPS catabolic pathway was identified in marine Roseobacteraceae strains, where HpsO and HpsP dehydrogenases at the gateway to DHPS catabolism act specifically on R-DHPS and S-DHPS, respectively. R-DHPS is also a substrate for the dehydrogenase HpsN. All three dehydrogenases generate stable hydrogen bonds between the chirality-center hydroxyls of DHPS and highly conserved residues, and HpsP also form coordinate-covalent bonds between the chirality-center hydroxyls and Zn2+, which determines the mechanistic basis of strict stereoselectivity. We further illustrated the role of enzymatic promiscuity in the evolution of DHPS metabolism in Roseobacteraceae and SAR11. This study provides the first evidence of chirality's involvement in phytoplankton-bacteria metabolic currencies, opening a new avenue for understanding the ocean organosulfur cycle.

PubMed: 38709871
DOI: 10.1093/ismejo/wrae084

実験手法

X-RAY DIFFRACTION (1.7 Å)