8WUV
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt)
Summary for 8WUV
Entry DOI | 10.2210/pdb8wuv/pdb |
EMDB information | 37861 |
Descriptor | DNA (51-MER), DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3'), CRISPR-associated endonuclease Cas9/Csn1, ... (6 entities in total) |
Functional Keywords | crispr-cas, rna binding protein-rna-dna complex, rna binding protein/rna/dna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 6 |
Total formula weight | 294784.57 |
Authors | Shuto, Y.,Nakagawa, R.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Nureki, O. (deposition date: 2023-10-21, release date: 2024-06-05, Last modification date: 2024-09-11) |
Primary citation | Shuto, Y.,Nakagawa, R.,Zhu, S.,Hoki, M.,Omura, S.N.,Hirano, H.,Itoh, Y.,Zhang, F.,Nureki, O. Structural basis for pegRNA-guided reverse transcription by a prime editor. Nature, 631:224-231, 2024 Cited by PubMed: 38811740DOI: 10.1038/s41586-024-07497-8 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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