8WU6

Structure of a Nerylneryl Diphosphate Synthase from Solanum lycopersicum

8WU6 の概要

• エントリーDOI: 10.2210/pdb8wu6/pdb
• 分子名称: Nerylneryl diphosphate synthase CPT2, chloroplastic (2 entities in total)
• 機能のキーワード: cis-prenyltransferase, biosynthetic protein
• 由来する生物種: Solanum lycopersicum (tomato)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73792.61

構造登録者

Li, F.R.,Wang, Q.L.,Pan, X.M.,Dong, L.B. (登録日: 2023-10-20, 公開日: 2024-05-08, 最終更新日: 2024-07-10)

主引用文献

Li, F.R.,Wang, Q.,Pan, X.,Xu, H.M.,Dong, L.B.
Discovery, Structure, and Engineering of a cis-Geranylfarnesyl Diphosphate Synthase.
Angew.Chem.Int.Ed.Engl., 63:e202401669-e202401669, 2024

PubMed Abstract: cis-Prenyltransferases (cis-PTs) catalyze the sequential head-to-tail condensation of isopentenyl diphosphate (IPP) to allylic diphosphates, producing mixed E-Z prenyl diphosphates of varying lengths; however, the specific enzymes synthesizing cis-C prenyl diphosphates have not been identified. Herein, we present the discovery and characterization of a cis-geranylfarnesyl diphosphate synthase (ScGFPPS) from Streptomyces clavuligerus. This enzyme demonstrates high catalytic proficiency in generating six distinct cis-polyisoprenoids, including three C and three C variants. We determined the crystal structure of ScGFPPS. Additionally, we unveil the crystal structure of nerylneryl diphosphate synthase (NNPS), known for synthesizing an all-cis-C polyisoprenoid. Comparative structural analysis of ScGFPPS and NNPS has identified key differences that influence product specificity. Through site-directed mutagenesis, we have identified eight single mutations that significantly refine the selectivity of ScGFPPS for cis-polyisoprenoids. Our findings not only expand the functional spectrum of cis-PTs but also provide a structural comparison strategy in cis-PTs engineering.

PubMed: 38651244
DOI: 10.1002/anie.202401669

実験手法

X-RAY DIFFRACTION (1.81 Å)