8WP0

Structure of the Arabidopsis E529Q/E1174Q ABCB19 in the ATP bound state

8WP0 の概要

• エントリーDOI: 10.2210/pdb8wp0/pdb
• EMDBエントリー: 37705
• 分子名称: ABC transporter B family member 19, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: abc transporter, transport protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 137993.57

構造登録者

Ying, W.,Wei, H.,Liu, X.,Sun, L. (登録日: 2023-10-08, 公開日: 2024-03-13, 最終更新日: 2025-09-17)

主引用文献

Ying, W.,Wang, Y.,Wei, H.,Luo, Y.,Ma, Q.,Zhu, H.,Janssens, H.,Vukasinovic, N.,Kvasnica, M.,Winne, J.M.,Gao, Y.,Tan, S.,Friml, J.,Liu, X.,Russinova, E.,Sun, L.
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Science, 383:eadj4591-eadj4591, 2024

PubMed Abstract: Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In , ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.

PubMed: 38513023
DOI: 10.1126/science.adj4591

実験手法

ELECTRON MICROSCOPY (3.4 Å)