8WNF

Crystal structure of H. pylori isoleucyl-tRNA synthetase (HpIleRS) in apo form

8WNF の概要

• エントリーDOI: 10.2210/pdb8wnf/pdb
• 分子名称: Isoleucine--tRNA ligase, GLYCEROL, ZINC ION, ... (5 entities in total)
• 機能のキーワード: isoleucyl-trna synthetase, helicobacter pylori, hpilers, ilers, aminoacylation, ligase
• 由来する生物種: Helicobacter pylori (Campylobacter pylori)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 107521.90

構造登録者

Guo, Y.,Li, S.,Zhang, T. (登録日: 2023-10-05, 公開日: 2024-02-14, 最終更新日: 2024-10-30)

主引用文献

Chen, X.,Guo, Y.,Shi, J.,Wang, Y.,Guo, X.,Wu, G.,Li, S.,Zhang, T.
Structural basis for substrate and antibiotic recognition by Helicobacter pylori isoleucyl-tRNA synthetase.
Febs Lett., 598:521-536, 2024

PubMed Abstract: Helicobacter pylori infection is a global health concern, affecting over half of the world's population. Acquiring structural information on pharmacological targets is crucial to facilitate inhibitor design. Here, we have determined the crystal structures of H. pylori isoleucyl-tRNA synthetase (HpIleRS) in apo form as well as in complex with various substrates (Ile, Ile-AMP, Val, and Val-AMP) or an inhibitor (mupirocin). Our results provide valuable insights into substrate specificity, recognition, and the mechanism by which HpIleRS is inhibited by an antibiotic. Moreover, we identified Asp641 as a prospective regulatory site and conducted biochemical analyses to investigate its regulatory mechanism. The detailed structural information acquired from this research holds promise for the development of highly selective and effective inhibitors against H. pylori infection.

PubMed: 38246751
DOI: 10.1002/1873-3468.14805

実験手法

X-RAY DIFFRACTION (1.9 Å)