8WMH

Structure of CbCas9 bound to 6-nucleotide complementary DNA substrate

8WMH の概要

• エントリーDOI: 10.2210/pdb8wmh/pdb
• EMDBエントリー: 37652
• 分子名称: deadCbCas9, sgRNA, TS, ... (4 entities in total)
• 機能のキーワード: cas9 complex, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• 由来する生物種: Chryseobacterium; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 228123.45

構造登録者

Zhang, S.,Lin, S.,Liu, J.J.G. (登録日: 2023-10-03, 公開日: 2024-06-05, 最終更新日: 2025-06-25)

主引用文献

Zhang, S.,Sun, A.,Qian, J.M.,Lin, S.,Xing, W.,Yang, Y.,Zhu, H.Z.,Zhou, X.Y.,Guo, Y.S.,Liu, Y.,Meng, Y.,Jin, S.L.,Song, W.,Li, C.P.,Li, Z.,Jin, S.,Wang, J.H.,Dong, M.Q.,Gao, C.,Chen, C.,Bai, Y.,Liu, J.G.
Pro-CRISPR PcrIIC1-associated Cas9 system for enhanced bacterial immunity.
Nature, 630:484-492, 2024

PubMed Abstract: The CRISPR system is an adaptive immune system found in prokaryotes that defends host cells against the invasion of foreign DNA. As part of the ongoing struggle between phages and the bacterial immune system, the CRISPR system has evolved into various types, each with distinct functionalities. Type II Cas9 is the most extensively studied of these systems and has diverse subtypes. It remains uncertain whether members of this family can evolve additional mechanisms to counter viral invasions. Here we identify 2,062 complete Cas9 loci, predict the structures of their associated proteins and reveal three structural growth trajectories for type II-C Cas9. We found that novel associated genes (NAGs) tended to be present within the loci of larger II-C Cas9s. Further investigation revealed that CbCas9 from Chryseobacterium species contains a novel β-REC2 domain, and forms a heterotetrameric complex with an NAG-encoded CRISPR-Cas-system-promoting (pro-CRISPR) protein of II-C Cas9 (PcrIIC1). The CbCas9-PcrIIC1 complex exhibits enhanced DNA binding and cleavage activity, broader compatibility for protospacer adjacent motif sequences, increased tolerance for mismatches and improved anti-phage immunity, compared with stand-alone CbCas9. Overall, our work sheds light on the diversity and 'growth evolutionary' trajectories of II-C Cas9 proteins at the structural level, and identifies many NAGs-such as PcrIIC1, which serves as a pro-CRISPR factor to enhance CRISPR-mediated immunity.

PubMed: 38811729
DOI: 10.1038/s41586-024-07486-x

実験手法

ELECTRON MICROSCOPY (2.6 Å)