8WCL

FCP pentamer in Chaetoceros gracilis

8WCL の概要

• エントリーDOI: 10.2210/pdb8wcl/pdb
• EMDBエントリー: 37442
• 分子名称: Chlorophyll a/c-binding protein Lhcf6, 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE, Chlorophyll a/b-binding protein, ... (10 entities in total)
• 機能のキーワード: fcp pentamer in chaetoceros gracilis, photosynthesis
• 由来する生物種: Chaetoceros neogracilis; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 171181.23

構造登録者

Feng, Y.,Li, Z.,Zhou, C.,Liu, C.,Shen, J.-R.,Wang, W. (登録日: 2023-09-12, 公開日: 2024-07-31, 最終更新日: 2024-11-27)

主引用文献

Zhou, C.,Feng, Y.,Li, Z.,Shen, L.,Li, X.,Wang, Y.,Han, G.,Kuang, T.,Liu, C.,Shen, J.R.,Wang, W.
Structural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states.
Plant Commun., 5:101041-101041, 2024

PubMed Abstract: Diatoms, a group of prevalent marine algae, significantly contribute to global primary productivity. Their substantial biomass is linked to enhanced absorption of blue-green light underwater, facilitated by fucoxanthin chlorophyll a/c-binding proteins (FCPs), exhibiting oligomeric diversity across diatom species. Utilizing mild CN-PAGE analysis on solubilized thylakoid membranes, we displayed monomeric, dimeric, trimeric, tetrameric and pentameric FCPs in diatoms. Mass spectrometry analysis revealed each oligomeric FCP has specific protein compositions, constituting a large Lhcf family of FCP antennas. In addition, we resolved the structures of Thalassiosira pseudonana FCP (Tp-FCP) homotrimer and Chaetoceros gracilis FCP (Cg-FCP) pentamer by cryo-electron microscopy at 2.73 Å and 2.65 Å resolutions, respectively. The distinct pigment composition and organization in various oligomeric FCPs change their blue-green light-harvesting, excitation energy transfer pathways. In comparison to dimeric and trimeric FCPs, Cg-FCP tetramer and Cg-FCP pentamer exhibit stronger absorption by Chls c, red-shifted and broader Chl a fluorescence emission, as well as more robust circular dichroism signals originating from Chl a-carotenoid dimers. These spectroscopic characteristics indicate that Chl a molecules in Cg-FCP tetramer and Cg-FCP pentamer are more heterogeneous than in both dimers and Tp-FCP trimer. The structural and spectroscopic insights provided by this study contribute to a better understanding of the mechanisms that empower diatoms to adapt to fluctuating light environments.

PubMed: 39030906
DOI: 10.1016/j.xplc.2024.101041

実験手法

ELECTRON MICROSCOPY (2.65 Å)