8W5D

Cryo-EM structure of Qb-Ab1

8W5D の概要

• エントリーDOI: 10.2210/pdb8w5d/pdb
• EMDBエントリー: 37286
• 分子名称: Heavy chain of Ab1, Minor capsid protein A1, Light chain of Ab1 (3 entities in total)
• 機能のキーワード: qb, cryo-em, antibody, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53937.16

構造登録者

Bao, K.Y.,Li, R.H.,Hua, Z.L.,Hou, B.D.,Zhu, P. (登録日: 2023-08-26, 公開日: 2025-02-26)

主引用文献

Li, R.,Bao, K.,Liu, C.,Ma, X.,Hua, Z.,Zhu, P.,Hou, B.
Competition propels, rather than limits, the success of low-affinity B cells in the germinal center response.
Cell Rep, 44:115334-115334, 2025

PubMed Abstract: The germinal center (GC) sets an environment where antigen-specific B cells are compelled to continuously increase their affinity to compete for the antigen and obtain Tfh help for survival and propagation. Previous studies indicated that low-affinity B cells are disadvantaged in the presence of high-affinity ones, suggesting that competition may lead to the elimination of low-affinity B cells and their descendants. However, using a multivalent virus-mimicking antigen, our study demonstrates that low-affinity B cells not only successfully participate in GC responses alongside high-affinity B cells but also undergo accelerated affinity maturation under the more stringent competition. Furthermore, our cryo-electron-microscopy-based structural analysis reveals that both low-affinity and high-affinity B cells compete for the same antigenic epitope. Although the applicability of this idealized GC competition to true pathogen-induced responses remains uncertain, this change in perspective on the role of competition in low-affinity B cell evolution provides valuable insights for vaccine development.

PubMed: 39955776
DOI: 10.1016/j.celrep.2025.115334

実験手法

ELECTRON MICROSCOPY (4.3 Å)