8W1C

Cryo-EM structure of BTV pre-subcore

8W1C の概要

• エントリーDOI: 10.2210/pdb8w1c/pdb
• EMDBエントリー: 43719
• 分子名称: Core protein VP3, VP6 (2 entities in total)
• 機能のキーワード: bluetongue virus, capsid protein, pre-subcore, assembly intermediate, viral protein
• 由来する生物種: Bluetongue virus (serotype 1 / isolate South Africa); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 1212068.44

構造登録者

Xia, X.,Sung, P.Y.,Martynowycz, M.W.,Gonen, T.,Roy, P.,Zhou, Z.H. (登録日: 2024-02-15, 公開日: 2024-04-24, 最終更新日: 2024-05-08)

主引用文献

Xia, X.,Sung, P.Y.,Martynowycz, M.W.,Gonen, T.,Roy, P.,Zhou, Z.H.
RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.
Cell, 187:2236-2249.e17, 2024

PubMed Abstract: Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 Å, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond.

PubMed: 38614100
DOI: 10.1016/j.cell.2024.03.007

実験手法

ELECTRON MICROSCOPY (3.6 Å)