8VXQ

Cryo-EM structure of phage DEV ejection proteins gp72:gp73

8VXQ の概要

• エントリーDOI: 10.2210/pdb8vxq/pdb
• EMDBエントリー: 43629
• 分子名称: gp72, N4 gp52-like protein (2 entities in total)
• 機能のキーワード: phage, bacteriophage, structural protein, viral protein, outer membrane protein, gp73, gp74, dev
• 由来する生物種: Pseudomonas phage vB_PaeP_DEV; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 664403.87

構造登録者

Iglesias, S.M.,Cingolani, G. (登録日: 2024-02-05, 公開日: 2024-04-03, 最終更新日: 2024-10-16)

主引用文献

Lokareddy, R.K.,Hou, C.D.,Forti, F.,Iglesias, S.M.,Li, F.,Pavlenok, M.,Horner, D.S.,Niederweis, M.,Briani, F.,Cingolani, G.
Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.
Nat Commun, 15:8482-8482, 2024

PubMed Abstract: DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae.

PubMed: 39353939
DOI: 10.1038/s41467-024-52752-1

実験手法

ELECTRON MICROSCOPY (3.1 Å)